biotin-labeled rabbit anti-human bdnf polyclonal antibodies  (PeproTech)

Journal: World Journal of Surgical Oncology

Article Title: Association of BDNF and BMPR1A with clinicopathologic parameters in benign and malignant gallbladder lesions

doi: 10.1186/1477-7819-11-80

Figure Lengend Snippet: Expression of brain-derived neurotrophic factor (BDNF) and bone morphogenetic protein receptor type IA (BMPR1A) in gallbladder adenocarcinoma, peritumoral, adenoma, polyp, and chronic cholecystitis tissues

Article Snippet: Immunohistochemical staining was carried out using rabbit anti-human BDNF and BMPR1A polyclonal antibodies (gift from Abgent Company, San Diego CA, USA) and a commercial kit (EnVision TM Detection Kit; Dako Laboratories, Carpinteria, CA, USA) in accordance with the manufacturer’s protocol (ChemMate TM EnVision+/HRP/DAB).

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Association of BDNF and BMPR1A with clinicopathologic parameters in benign and malignant gallbladder lesions

doi: 10.1186/1477-7819-11-80

Figure Lengend Snippet: Expression of brain-derived neurotrophic factor (BDNF) and bone morphogenetic protein receptor, type IA (BMPR1A) in gallbladder adenocarcinoma, peritumoral, adenoma, polyp, and chronic cholecystitis tissues.

Article Snippet: Immunohistochemical staining was carried out using rabbit anti-human BDNF and BMPR1A polyclonal antibodies (gift from Abgent Company, San Diego CA, USA) and a commercial kit (EnVision TM Detection Kit; Dako Laboratories, Carpinteria, CA, USA) in accordance with the manufacturer’s protocol (ChemMate TM EnVision+/HRP/DAB).

Techniques: Expressing, Derivative Assay

Journal: World Journal of Surgical Oncology

Article Title: Association of BDNF and BMPR1A with clinicopathologic parameters in benign and malignant gallbladder lesions

doi: 10.1186/1477-7819-11-80

Figure Lengend Snippet: Expression of brain-derived neurotrophic factor (BDNF) and bone morphogenetic protein receptor type IA (BMPR1A) and their correlation with the clinicopathologic parameters of gallbladder adenocarcinoma

Article Snippet: Immunohistochemical staining was carried out using rabbit anti-human BDNF and BMPR1A polyclonal antibodies (gift from Abgent Company, San Diego CA, USA) and a commercial kit (EnVision TM Detection Kit; Dako Laboratories, Carpinteria, CA, USA) in accordance with the manufacturer’s protocol (ChemMate TM EnVision+/HRP/DAB).

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Association of BDNF and BMPR1A with clinicopathologic parameters in benign and malignant gallbladder lesions

doi: 10.1186/1477-7819-11-80

Figure Lengend Snippet: Expression of brain-derived neurotrophic factor (BDNF) and bone morphogenetic protein receptor type IA (BMPR1A) and their correlation with the clinicopathologic parameters of gallbladder adenocarcinoma.

Article Snippet: Immunohistochemical staining was carried out using rabbit anti-human BDNF and BMPR1A polyclonal antibodies (gift from Abgent Company, San Diego CA, USA) and a commercial kit (EnVision TM Detection Kit; Dako Laboratories, Carpinteria, CA, USA) in accordance with the manufacturer’s protocol (ChemMate TM EnVision+/HRP/DAB).

Techniques: Expressing, Derivative Assay

Journal: World Journal of Surgical Oncology

Article Title: Association of BDNF and BMPR1A with clinicopathologic parameters in benign and malignant gallbladder lesions

doi: 10.1186/1477-7819-11-80

Figure Lengend Snippet: Relationships between brain-derived neurotrophic factor (BDNF) and bone morphogenetic protein receptor type IA (BMPR1A) expression and clinicopathologic characteristics and average survival of patients with gallbladder adenocarcinoma

Article Snippet: Immunohistochemical staining was carried out using rabbit anti-human BDNF and BMPR1A polyclonal antibodies (gift from Abgent Company, San Diego CA, USA) and a commercial kit (EnVision TM Detection Kit; Dako Laboratories, Carpinteria, CA, USA) in accordance with the manufacturer’s protocol (ChemMate TM EnVision+/HRP/DAB).

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Association of BDNF and BMPR1A with clinicopathologic parameters in benign and malignant gallbladder lesions

doi: 10.1186/1477-7819-11-80

Figure Lengend Snippet: Multivariate Cox regression analysis of disease-specific survival in 65 patients who underwent surgical resection of gallbladder carcinoma

Article Snippet: Immunohistochemical staining was carried out using rabbit anti-human BDNF and BMPR1A polyclonal antibodies (gift from Abgent Company, San Diego CA, USA) and a commercial kit (EnVision TM Detection Kit; Dako Laboratories, Carpinteria, CA, USA) in accordance with the manufacturer’s protocol (ChemMate TM EnVision+/HRP/DAB).

Techniques:

Journal: Journal of Microbiology and Biotechnology

Article Title: Korean Red Pine ( Pinus densiflora ) Bark Extract Attenuates Aβ-Induced Cognitive Impairment by Regulating Cholinergic Dysfunction and Neuroinflammation

doi: 10.4014/jmb.2207.07015

Figure Lengend Snippet: ( A ) Western blot band image, Protein expression levels of ( B ) p-CREB, and ( C ) BDNF. The results shown are mean ± SD ( n = 3). Data were statistically considered at p < 0.05, and different small letters represent statistical difference.

Article Snippet: Brain-derived neurotrophic factor (BDNF) (CSB-PA05775A0Rb) was purchased from Cusabio Biotech (China), and anti-phospho-cAMP response element-binding protein (p-CREB) (9198) and anti-rabbit (7074S) were purchased from Cell Signaling Technology (USA).

Techniques: Western Blot, Expressing

Journal: BioMed Research International

Article Title: Full-Length TrkB Variant in NSCLC Is Associated with Brain Metastasis

doi: 10.1155/2020/4193541

Figure Lengend Snippet: Immunohistochemical detection of TrkB and BDNF protein expression in sections of primitive ADKs of the lung with (Group 1) or without (Group 2) brain metastasis. The histograms summarize the results obtained. A statistically significant increase of TrkB protein expression was observed in samples from primitive ADK in Group 1 compared with Group 2. The BDNF protein expression was higher in Group 2 in comparison with Group 1. Notice the anatomical localization of BDNF and TrkB protein expression in ADK cell. BDNF antibodies generated a prevalent nuclear dark brown immunoreaction. The TrkB antibody generated a cytoplasmic and cell membrane immunoreaction. A clear and specific TrkB and BDNF immunostaining was documented in ADKs cells in both primitive and brain metastasis in Group 1. Contrarily, in Group 2, a strong and specific BDNF immunostaining was documented in brain metastasis, but not for TrkB. The data are the mean + SD of different experiments performed in triplicate. ×20 ∗ p < 0.0177.

Article Snippet: Histological sections fixed in formalin and included in paraffin were immunostained with the following antibodies: rabbit anti-human TrkB antibody (sc-12 rabbit polyclonal affinity purified antibody raised against a peptide mapping within the C-terminal cytoplasmic domain of TrkB of mouse origin, Santa Cruz Biotechnology, Dallas, Texas USA) dilution 1 : 500; rabbit anti-human -BDNF polyclonal antibody (H20, sc-548; epitope mapping at the N-terminus of the mature chain of mature NGF of human origin, Santa Cruz Biotechnology, Dallas, Texas USA) dilution 1 : 1000 dilution.

Techniques: Immunohistochemical staining, Expressing, Comparison, Generated, Membrane, Immunostaining

Journal: BioMed Research International

Article Title: Full-Length TrkB Variant in NSCLC Is Associated with Brain Metastasis

doi: 10.1155/2020/4193541

Figure Lengend Snippet: Immunohistochemical detection of TrkB and BDNF protein expression in section samples obtained by Group 1 (ADK with brain metastasis). (a) The histograms summarize the results obtained. A statistical significant increase of TrkB and BDNF protein expression was observed in samples from metastasis compared with relative primitive ADKs. (b) Notice the anatomical localization of BDNF and TrkB protein expression in ADK cells. A strong specific immunostaining was clearly documented in both primitive ADKs and brain metastasis (a–d). The data are the mean ± SD of different experiments performed in triplicate. ADK, primitive ADKs; BM, corresponding brain metastasis. ∗ p < 0.02; ∗∗ p < 0.004.

Article Snippet: Histological sections fixed in formalin and included in paraffin were immunostained with the following antibodies: rabbit anti-human TrkB antibody (sc-12 rabbit polyclonal affinity purified antibody raised against a peptide mapping within the C-terminal cytoplasmic domain of TrkB of mouse origin, Santa Cruz Biotechnology, Dallas, Texas USA) dilution 1 : 500; rabbit anti-human -BDNF polyclonal antibody (H20, sc-548; epitope mapping at the N-terminus of the mature chain of mature NGF of human origin, Santa Cruz Biotechnology, Dallas, Texas USA) dilution 1 : 1000 dilution.

Techniques: Immunohistochemical staining, Expressing, Immunostaining

Journal: BioMed Research International

Article Title: Full-Length TrkB Variant in NSCLC Is Associated with Brain Metastasis

doi: 10.1155/2020/4193541

Figure Lengend Snippet: Immunohistochemical detection of BDNF protein expression in section of metastasis from the skin, liver, and bone. The BDNF immunostaining was strongly expressed in the different metastatic sites studied (a, b). Note the specific clear immunostaining within cancer cells in metastatic cancer cells. ×20.

Article Snippet: Histological sections fixed in formalin and included in paraffin were immunostained with the following antibodies: rabbit anti-human TrkB antibody (sc-12 rabbit polyclonal affinity purified antibody raised against a peptide mapping within the C-terminal cytoplasmic domain of TrkB of mouse origin, Santa Cruz Biotechnology, Dallas, Texas USA) dilution 1 : 500; rabbit anti-human -BDNF polyclonal antibody (H20, sc-548; epitope mapping at the N-terminus of the mature chain of mature NGF of human origin, Santa Cruz Biotechnology, Dallas, Texas USA) dilution 1 : 1000 dilution.

Techniques: Immunohistochemical staining, Expressing, Immunostaining